The recognition that microbes are the life support system of the biosphere and play a major role in the health of all life forms provides us with a unique opportunity to channel resources into utilizing them for our benefit. This policy document was prepared by assessing the scientific rationale and evidence for the application of beneficial microbes to One Health. Ten recommendations are made including the establishment of a new national program that creates strategies, provides funding, and partners with industries and end-users to make Canada a self-directed global leader in this transformative field. Issues covered include biotics, fecal microbiota transplant, fundamental and applied research, regulatory, the education system, and applications to pollinators, aquaculture, coral, biofertilizers, livestock, companion animals, horses, and sustainability of agriculture and environmental management. It is hoped that this document will provide politicians, bureaucrats, academics, and representatives of the end users of beneficial microbial products the incentive to appoint an expert panel to develop the means to implement a program of this type. At a time when climate change, biodiversity, long-term health, integration of cultures, and access to home resourced nutritious food are high on government agendas, the proposed program offers a novel means to positively influence residents across the country.